Corrosion Behaviour and J774A.1 Macrophage Response to Hyaluronic Acid Functionalization of Electrochemically Reduced Graphene Oxide on Biomedical Grade CoCr
نویسندگان
چکیده
Improvements in the lubrication of metal–metal joint prostheses are great clinical interest order to minimize particles released during wear–corrosion processes. In this work, electrochemically reduced graphene oxide (ErGO) on CoCr was functionalized with hyaluronic acid (ErGOHA). Functionalization carried out by soaking for 24 h phosphate buffer saline (PBS) solution containing 3 g/L (HA). The corrosion performance CoCrErGO and CoCrErGOHA surfaces studied electrochemical impedance spectroscopy (EIS) 7 days PBS. Biocompatibility cytotoxicity were mouse macrophages J774A.1 cell line measurement mitochondrial activity (WST-1 assay) plasma membrane damage (LDH assay). inflammatory response examined through TNF-? IL-10 cytokines culture supernatants, used as indicators pro-inflammatory anti-inflammatory responses, respectively. EIS diagrams revealed two time constants: first one, attributed hydration diffusion processes HA layer adsorbed ErGO, second resistance ErGOHA/CoCr interface. Macrophage assays showed better behavior than based their biocompatible, cytotoxic, responses. Comparative analysis that functionalization induces higher values cytokine, suggesting an improvement behavior.
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ژورنال
عنوان ژورنال: Metals
سال: 2021
ISSN: ['2075-4701']
DOI: https://doi.org/10.3390/met11071078